![]() ![]() Purdue Research Foundation and USDA-ARSīaker JC, Steele C, Dure L III (1988) Sequence and characterization of six Lea proteins and their genes from cotton. This process is experimental and the keywords may be updated as the learning algorithm improves.Īrgos P (1989) Editbase, computer program. These keywords were added by machine and not by the authors. Furthermore, the stress-induced expression of these genes was found initially in non-seed tissues. In most all cases, the high expression of these genes was found to be up-regulated by exogenous ABA as well. Many were uncovered in studies designed to find genes whose expression is up-regulated in response to water stress, salt stress and cold stress. In the last several years a large number of gene sequences have been reported that are homologous to the sequenced lea gene families. By 1988 a representative gene from each of six different lea families had been sequenced and their amino acid sequence deduced (Baker et al. To reflect the developmental period of high accumulation, these protein families were named Lea, mnemonic for late embryogenesis abundant likewise lea genes and mRNAs. ![]() Subsequently, cDNA representatives of many of these protein families were obtained by subtractive hybridization and identified by hybrid arrested translation (Galau et al. ![]() These proteins could be induced to accumulate earlier in embryogenesis when immature embryos were dissected and incubated in dilute solutions of ABA (without ABA, the embryos germinate precociously). Over 10 years ago several families of proteins that accumulate to high levels during the maturation phase of cotton embryogenesis were identified by 2D gel electrophoresis (Dure et al. ![]()
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